The present document describes a standard technique that allows establishing species and biovars of an identified Brucella culture. Species identification is based on two main sets of properties: lysis by phages and oxidative metabolic profiles on selected amino acid and carbohydrate substrates. The described identification techniques enable to establish species and biovar of specific Brucella culture.

The present document describes a standard technique that allows research and bacteriological identification of the Brucella genus, in animal samples (ruminants, equidae, suidae, camelidae and carnivores, marine mammals, both wild and domestic). This is an isolation technique on solid culture agar medium. The described identification techniques enable a presumptive identification of bacteria of the Brucella genus.