Characterisation/Epidemiology

English

Enquiry of typing methods for Listeria monocytogenes

Dear Colleagues,

Please find enclosed :

-          Circular Letter 2014/01,
-          the link of the enquiry to be filled out ideally by 25/01/2014 but before 10/02/2014:
 https://www.enquetes.anses.fr/formation2/EURLLm-InventoryoftypingmethodsforListeria/questionnaire.htm?aspxid=BsSLGg7G/xqCAhsVKtWvQsiaLJDxom+k&&key=FKI-BFMALDFSRD&auto=1

English

LISTERIA MONOCYTOGENES MOLECULAR SEROTYPING, DETERMINATION OF THE SEROGROUP

This document describes the method for PCR serotyping of Listeria
monocytogenes. This molecular method is based on the amplification of specific region on
the genome of Listeria monocytogenes. Depending on the serogroups, these regions are
present or absent. An amplification by multiplex PCR of the genetic markers: lmo1118,
lmo0737, orf2110, orf2819 is performed. A sequence specific of Listeria genus located on the
prs gene (phosphoribosylpyrophosphate synthetase) and a specific sequence of the species
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