The Real Time Polymerase Chain Reaction (PCR) or Quantitative PCR is a molecular diagnostic tool used to detect nucleic acids, and in this case Brucella DNA (Bounaadja et al., 2009).

Principle

The present document describes a standard technique aiming at real-time Polymerase Chain Reaction (PCR) to detect the presence / absence of Brucella DNA in animal / human samples. Prior to PCR, the DNA extraction protocols have to be validated for each type of sample handled in the laboratory in order to assure standardized performances. The ID Gene® Brucella spp Triplex (IDBRU) kit is a Reel-time Polymerase Chain Reaction (Rt-PCR) kit for specific Brucella spp. sequence amplification based on TaqMan® technology. This is a qualitative Triplex system that enables simultaneous amplification of the target DNA and internal endogenous and exogenous controls.

Scientific references
HOLZAPFEL M., 2018. De l’épidémiologie moléculaire aux analyses fonctionnelles de Brucella chez les ruminants, une approche intégrée pour l’identification et l’étude de la diversité phénotypique d’un genre génétiquement homogène. Thèse de doctorat en Microbiologie, soutenue le 26-11-2018 à Paris Est, dans le cadre de l’École doctorale ABIES, 200 pages. Online document: http://www.theses.fr/2018PESC1141/document