EURL
European Union Reference Laboratory for
Foot-and-Mouth disease
Methods
The EURL for FMD, among other missions, gathers data and information on the methods of diagnosis and differential diagnosis used in the different National Laboratories.
A list of methods inspired by the WOAH official methods is available on this website.
This kit is used for the sequencing of the whole genome of Foot-and-Mouth Disease Virus.
This procedure describes the sequencing of the VP1 gene of FMDV using a Sanger sequencing approach and the SeqStudio Genetic Analyzer from Life technologies.
This SOP describes the procedure for the detection of Senecavirus A (SVA) RNA using a (triplex) real-time RT-PCR.
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus topotype SAT2/V.
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus topotype O/EA-3 (from West and North Africa).
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus lineage O/ME-SA/SA-2018, as it is an emerging lineage in South Asia since 2018.
All methods used for FMD detection, including commercial kits, must be WOAH-based methods validated according to international standards.
You will find attached the most recent version of the WOAH terrestrial manual chapter regarding foot-and-mouth disease.
Diagnostic methods for FMD detection and typing are described in the WOAH Terrestrial Manual.
This document sums up the diagnostic strategy as a decision tree describing the workflow for implementation of the different methods prescribed.
All methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
This SOP is the WOAH-based method currently used at the EURL for FMD.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
This SOP is the WOAH-based method currently used at the EURL for FMD detection.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.