EURL rabies
Collection of samples, techniques, validation and interpretation of the diagnostic methods for the purposes of rabies surveillance are presented here.
Diagnostic methods considered compliant are presented in the WOAH rabies manual. A list of procedures inspired by the WOAH official methods is also proposed.
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Annual inter-laboratory test for rabies diagnosis - Year 2015
♦ 1st June 2015 ♦
A panel of 9 brain samples was sent on 1st June to 47 participating laboratories. Please do not forget to send back your results before 1st July and to fill in the online technical questionnaires. Should you encounter any problems with the reception of the parcel or with the application of the test, do not hesitate to contact us. We wish you all a good test!
Workshop for rabies 2015
♦ 27-28 May 2015 ♦
The rabies EURL annual meeting gathered a total of 52 people. Many thanks and all our gratitude to our colleagues from the Croatian Veterinary Institute and to the Veterinary and Food Safety Directorate who made this event possible and successful. We also would like to warmly thank the European Commission and TAIEX for their support for travel and accommodation, as well as all the attendants and speakers for their contribution in this annual date. A Croatian veterinary newspaper article dedicated to this workshop is available here. This meeting gave the opportunity of many exchanges between attendants. As usual, a booklet with the abstracts of the talks was provided to every participant. All presentations will be soon on line in the restricted access part of the website. A photo souvenir is available here. Attendants eligible for the EC financial support have to send their original documents before 12 June.
Croatian National Reference Laboratory for Rabies
♦ 03 April 2015 ♦
The Laboratory for Rabies is one of the three laboratories in the department of virology at Croatian Veterinary Institute. Since 2011 Laboratory is recognized as a National Reference Laboratory for Rabies. The laboratory is also a EU approved laboratory for serological testing regarding rabies in dogs, cats and ferrets in the frame of pets travel. Main laboratory routine activities are connected with rabies surveillance and rabies monitoring programs. Monitoring program is conducted as a part of oral vaccination campaigns which has been started in 2011. During 2014 around 1000 samples have been tested as a part of surveillance program and around 3000 samples as part of monitoring program. The last rabies positive case has been recorded in February 2014.
Methods
In laboratory the following methods are used:
| o | Fluorescence antibody test (FAT), |
| o | Fluorescence virus neutralisation test (FAVN), |
| o | Tissue culture infective test (TCIT), |
| o | Molecular techniques (RT-PCR and QRT-PCR), |
| o | Complete genome sequencing, |
| o | OTC determination, |
| o | Age determination |
| o | Baits titration. |
FAT and FAVN methods are accredited according to ISO17025.
Fields of research
Laboratory Research activities are mainly connected with rabies particularly in the field of Rabies epidemiology and diagnostic techniques. Recently, the research regarding stem cells and viral metagenomic were ongoing.
Staff
The Laboratory represented by six agents is headed by Dr Tomislav Bedeković:
- Ivana Lojkić, Scientist; PhD in molecular biology, Deputy Head
- Nina Krešić, Doctor of Veterinary Medicine
- Ivana Šimić, Doctor of Veterinary Medicine;
- Ines Ugrin, Technician
- Mirjana Frljužec, Technician
Recent rabies publication:
- Bedeković, T., Lemo N, Lojkić I, Mihaljević Z, Jungić A, Cvetnić Z, Cač Z, Hostnik P. (2013). Modification of the fluorescent antibody virus neutralisation test - elimination of the cytotoxic effect for the detection of rabies virus neutralising antibodies. Journal of Virological Methods. 189, 204-208.
- Lojkić, Ivana; Bedeković, Tomislav; Čač, Željko; Lemo, Nina; Cvetnić, Željko (2013). Clinical rabies in cattle imported into Croatia. Veterinary record. 172, 22-23.
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Lojkić, Ivana; Čač, Željko; Bedeković, Tomislav; Lemo, Nina; Brstilo, Mate; Müller, Thomas; Freuling, Conrad M. (2012). Diversity of currently circulating rabies virus strains in Croatia. Berliner und Münchener tierärztliche Wochenschrift. 125, 249-254.
World Veterinary Day Award 2015
♦ 30 April 2015 ♦
[Relayed from the OIE website] World Veterinary Day was instigated by the World Veterinary Association (WVA) in 2000 to be celebrated annually on the last Saturday of April. In 2008 the WVA and the World Organisation for Animal Health (OIE) agreed on the creation of the World Veterinary Day Award aimed at rewarding the most successful celebration of the contribution of the veterinary profession to society. The winner of the 2015 Award will be announced at the Opening Ceremony of the OIE 83rd General Session to be held in Paris, France on 24 May 2015 and will be invited to the 32nd World Veterinary Congress, 13-17 September in Istanbul, Turkey where he/she will receive the price of 1.000 US$. The competition is open to all WVA veterinary associations, alone, or in cooperation with any other selected veterinary body. The selected theme for 2015 are the vector-borne diseases with a zoonotic potential. More info on http://www.oie.int/en/for-the-media/press-releases/detail/article/world-veterinary-day-award-2015/